Flow cytometric analysis of virus-infected cells and its potential use for screening antiviral agents.
J Virol Methods
; 27(3): 241-52, 1990 Mar.
Article
em En
| MEDLINE
| ID: mdl-1691199
ABSTRACT
Virus-infected cells were analyzed using multiparameter flow cytometry. Two virus-cell systems were investigated HSV-1-infected VF cells and influenza C virus JHB/1/66-infected MDCK cells. Analysis included the measurement of the appearance of virus specific antigens. On individual cells, with polyclonal antibodies, antigens were first detected at 12 h p.i., and the numbers of labeled cells were followed up to 96 h p.i. The efficacy of four antiviral agents was tested with this system. The results were in good agreement with those of plaque reduction tests and indicated that this new method may be extremely useful for the correlation of viral and cellular events with antiviral activity. Finally, it was demonstrated that infected cells in both systems have a considerably greater volume than non-infected cells.
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Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Avaliação Pré-Clínica de Medicamentos
/
Citometria de Fluxo
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article