G-rich, a Drosophila selenoprotein, is a Golgi-resident type III membrane protein.
Biochem Biophys Res Commun
; 348(4): 1296-301, 2006 Oct 06.
Article
em En
| MEDLINE
| ID: mdl-16920070
ABSTRACT
G-rich is a Drosophila melanogaster selenoprotein, which is a homologue of human and mouse SelK. Subcellular localization analysis using GFP-tagged G-rich showed that G-rich was localized in the Golgi apparatus. The fusion protein was co-localized with the Golgi marker proteins but not with an endoplasmic reticulum (ER) marker protein in Drosophila SL2 cells. Bioinformatic analysis of G-rich suggests that this protein is either type II or type III transmembrane protein. To determine the type of transmembrane protein experimentally, GFP-G-rich in which GFP was tagged at the N-terminus of G-rich, or G-rich-GFP in which GFP was tagged at the C-terminus of G-rich, were expressed in SL2 cells. The tagged proteins were then digested with trypsin, and analyzed by Western blot analysis. The results showed that the C-terminus of the G-rich protein was exposed to the cytoplasm indicating it is a type III microsomal membrane protein. G-rich is the first selenoprotein identified in the Golgi apparatus.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Drosophila
/
Drosophila melanogaster
/
Selenoproteínas
/
Complexo de Golgi
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article