Digital quantification using amplified single-molecule detection.
Nat Methods
; 3(9): 725-7, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-16929318
ABSTRACT
We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae.
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Base de dados:
MEDLINE
Assunto principal:
Vibrio cholerae
/
DNA Bacteriano
/
DNA Circular
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article