In vitro culture of tobacco callus on medium containing peptone and phytate leads to growth improvement and higher genetic stability.
Plant Cell Rep
; 26(2): 145-52, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-16972097
ABSTRACT
Growth and genetic stability of Nicotiana tabacum L. callus were strongly improved by replacing the inorganic nitrogen and phosphorus of the Murashige and Skoog's medium by a soybean peptone and phytate, respectively. Cell proliferation after subcultivation on the modified medium was highly stimulated as evidenced by a strong biomass increase; this improvement was mainly due to the organic N source. In addition, while calluses grown under standard conditions displayed various cell sizes and DNA contents, subcultivation on the modified medium led to homogeneous cell size distribution and stable 4C-8C DNA contents through several subcultures. This improved genetic stability was due to replacement of inorganic P by phytate, provided the presence of peptone. Such new media composition could be useful for slow-growing cell suspensions or calluses.
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Base de dados:
MEDLINE
Assunto principal:
Peptonas
/
Ácido Fítico
/
Nicotiana
/
Meios de Cultura
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article