Role of the wbt locus of Francisella tularensis in lipopolysaccharide O-antigen biogenesis and pathogenicity.
Infect Immun
; 75(1): 536-41, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17030571
ABSTRACT
Francisella tularensis is a highly infectious bacterial pathogen, responsible for the zoonotic disease tularemia. We screened a bank of transposon insertion mutants of F. tularensis subsp. holarctica LVS for colony morphology alterations and selected a mutant with a transposon insertion in wbtA, the first gene of the predicted lipopolysaccharide O-antigen gene cluster. Inactivation of wbtA led to the complete loss of O antigen, conferred serum sensitivity, impaired intracellular replication, and severely attenuated virulence in the mouse model. Notably, this mutant afforded protection against a challenge against virulent LVS.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Antígenos O
/
Francisella tularensis
/
Genes Bacterianos
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article