Migration of zebrafish primordial germ cells: a role for myosin contraction and cytoplasmic flow.
Dev Cell
; 11(5): 613-27, 2006 Nov.
Article
em En
| MEDLINE
| ID: mdl-17084355
ABSTRACT
The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.
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Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
/
Quimiotaxia
/
Miosinas
/
Citoplasma
/
Proteínas de Peixe-Zebra
/
Células Germinativas
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article