Interleukin-1beta and interleukin-6 stimulate matrix metalloproteinase-9 secretion in cultured myenteric glia.
J Surg Res
; 137(1): 38-45, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17109889
ABSTRACT
Western blotting of culture media of myenteric glia stained positive for GFAP revealed increased secretion of matrix metalloproteinase-9 (MMP-9) in interleukin-1beta (IL-1beta) stimulated cells (10 ng/mL) versus control (142 +/- 19 versus 42 +/- 19, P < 0.05). Interleukin-6 (IL-6) stimulated cells also showed increased expression of MMP-9 (10 ng/mL) versus control (69 +/- 14 versus 4 +/- 2, P < 0.01). Control and cytokine-stimulated cells secreted MMP-2 constituitively. Gelatin zymography demonstrated that products were biologically active. Cytoplasmic staining for MMP-9 was detected in IL-1beta and IL-6-stimulated cells but was negligible in controls. Cultured myenteric glia are responsive to IL-1beta and IL-6 stimulation by secreting MMPs into the extracellular environment.
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Base de dados:
MEDLINE
Assunto principal:
Neuroimunomodulação
/
Neuroglia
/
Interleucina-6
/
Metaloproteinase 9 da Matriz
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Interleucina-1beta
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Plexo Mientérico
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article