[Nuclear transfer of goat somatic cells transgenic for human lactoferrin].
Yi Chuan
; 28(12): 1513-9, 2006 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-17138536
ABSTRACT
Transgenic animal mammary gland bioreactors are being used to produce recombinant proteins with appropriate post-translational modifications, and nuclear transfer of transgenic somatic cells is a more powerful method to produce mammary gland bioreactor. Here we describe efficient gene transfer and nuclear transfer in goat somatic cells. Gene targeting vector pGBC2LF was constructed by cloning human lactoferrin (LF) gene cDNA into exon 2 of the milk goat beta-casein gene, and the endogenous start condon was replaced by that of human LF gene. Goat fetal fibroblasts were transfected with linearized pGBC2LF and 14 cell lines were positive according to PCR and Southern blot. The transgenic cells were used as donor cells of nuclear transfer, and some of reconstructed embryos could develop to blastocyst in vitro.
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Base de dados:
MEDLINE
Assunto principal:
Cabras
/
Técnicas de Transferência de Genes
/
Técnicas de Transferência Nuclear
/
Lactoferrina
Idioma:
Zh
Ano de publicação:
2006
Tipo de documento:
Article