Methylation of histone H3 lysine-79 by Dot1p plays multiple roles in the response to UV damage in Saccharomyces cerevisiae.
DNA Repair (Amst)
; 6(3): 383-95, 2007 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-17267293
Various proteins have been found to play roles in both the repair of UV damaged DNA and heterochromatin-mediated silencing in the yeast Saccharomyces cerevisiae. In particular, factors that are involved in the methylation of lysine-79 of histone H3 by Dot1p have been implicated in both processes, suggesting a bipartite function for this modification. We find that a dot1 null mutation and a histone H3 point mutation at lysine-79 cause increased sensitivity to UV radiation, suggesting that lysine-79 methylation is important for efficient repair of UV damage. Epistasis analysis between dot1 and various UV repair genes indicates that lysine-79 methylation plays overlapping roles within the nucleotide excision, post-replication and recombination repair pathways, as well as RAD9-mediated checkpoint function. In contrast, epistasis analysis with the H3 lysine-79 point mutation indicates that the lysine-to-glutamic acid substitution exerts specific effects within the nucleotide excision repair and post-replication repair pathways, suggesting that this allele only disrupts a subset of the functions of lysine-79 methylation. The overall results indicate the existence of distinct and separable roles of histone H3 lysine-79 methylation in the response to UV damage, potentially serving to coordinate the various repair processes.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Raios Ultravioleta
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Dano ao DNA
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Proteínas Nucleares
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Histonas
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Histona-Lisina N-Metiltransferase
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Proteínas de Saccharomyces cerevisiae
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Lisina
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article