[Establishment of a transgenic cell model for preliminary screening of chemopreventive agents].
Sheng Wu Gong Cheng Xue Bao
; 23(1): 85-9, 2007 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-17366893
ABSTRACT
To develop a GFP transgenic cell model under the transcriptional control of TK promoter adjacent to which ARE enhancer was inserted. Synthetic oligonucleotide ARE motif was annealed and purified then inserted into pTK-GFP to construct the vector of pARE-TK-GFP. The TK and ARE-TK fragments were amplified by PCR and cloned into pEGFP-N1 to reconstruct eukaryotic expression vectors of pTK-GFP/Neo and pARE-TK-GFP/Neo. They were transfected into HepG2 cells and clones resistant G418 were isolated. PDTC and Lentinan were used to induce the cell levels of GFP and the fluorescence was measured using a fluorescence plate reader. The results showed that the induced level of GFP is significantly increased and have dose-dependeny in a certain range. This findings indicated that such a cell model offered a potential platform for preliminary screening of all kinds of natural or synthetic chemopreventive agents.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Ensaios de Seleção de Medicamentos Antitumorais
/
Proteínas de Fluorescência Verde
/
Antineoplásicos
Idioma:
Zh
Ano de publicação:
2007
Tipo de documento:
Article