Genetic characterization and mapping of major gene resistance to potato leafroll virus in Solanumtuberosum ssp. andigena.
Theor Appl Genet
; 114(6): 1051-8, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17394033
ABSTRACT
Major gene inheritance of resistance to Potato leafroll virus (PLRV) was demonstrated in a parthenogenic population derived from the highly resistant tetraploid andigena landrace, LOP-868. This major gene or chromosome region seems to control a single mechanism for resistance to infection and virus accumulation in this source. About 149 dihaploid lines segregated in a ratio of 107 resistant to 32 susceptible, fitting the expected ratio for inheritance of a duplex gene under random chromatid segregation. A tetraploid AFLP map was constructed using as reference the ultra high density (UHD) map. All AFLP markers associated with PLRV resistance mapped to the same linkage group. Map position was confirmed by analysis of previously-mapped SSR markers. Rl (adg) is located on the upper arm of chromosome V, at 1 cM from its most closely linked AFLP marker, E35M48.192. This marker will be used to develop allele-specific primers or a pair of flanking PCR-based markers for their use in marker assisted selection.
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Base de dados:
MEDLINE
Assunto principal:
Solanum tuberosum
/
Mapeamento Cromossômico
/
Genes de Plantas
/
Luteovirus
/
Imunidade Inata
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article