TRF2 overexpression diminishes repair of telomeric single-strand breaks and accelerates telomere shortening in human fibroblasts.
Mech Ageing Dev
; 128(4): 340-5, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17395247
ABSTRACT
Repair of single strand breaks in telomeric DNA is less efficient than in other genomic regions. This leads to an increased vulnerability of telomeric DNA towards damage induced by reactive oxygen species (ROS) and to accelerated telomere shortening under oxidative stress. The causes for the diminished repair efficacy in telomeres are unknown. We show here that overexpression of the telomere-binding protein TRF2 further reduces telomeric, but not genomic, single strand break repair. This suggests the possibility of strand break repair in telomeres being sterically hindered by the three-dimensional structure of the telomere DNA-protein complex and explains the effect of TRF2 on telomere shortening rates in telomerase-negative cells.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
/
Telômero
/
Proteínas Semelhantes à Proteína de Ligação a TATA-Box
/
Reparo do DNA
/
Quebras de DNA de Cadeia Simples
/
Fibroblastos
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article