Double-hydrogel substrate as a model system for three-dimensional cell culture.

ABSTRACT

When cultured on two-dimensional surfaces, most adherent cells show profound differences from those in their native habitats. In addition to chemical factors, it is likely that both physical parameters, such as substrate rigidity, and topographical factors, such as the asymmetry in integrin anchorage, play a major role in the differences. We have designed a simple culture system that provides flexible, adhesive substrates for both dorsal and ventral cell surfaces. Fibroblasts in this system show the spindle or stellate morphology found in native tissues. The ease of preparation, versatility, and optical quality of this model system should greatly facilitate the understanding of cellular behavior and functions in vivo.