Cross-neutralizing antibodies in rabbits immunized with HIV-1 gp160 purified from simian cells infected with a recombinant vaccinia virus.
AIDS Res Hum Retroviruses
; 7(10): 791-8, 1991 Oct.
Article
em En
| MEDLINE
| ID: mdl-1742074
ABSTRACT
A recombinant vaccinia virus in which the transcription of the human immunodeficiency virus type 1 (BRU isolate) env gene is driven by the 11K late vaccinia promoter yields about 10-fold higher amounts of gp160 env protein upon infection of monkey cells than does a recombinant in which gp160 is expressed using the 7.5K early-late promoter. The gp160 was purified from detergent lysates of infected cells by lentil lectin affinity chromatography followed by immunoaffinity chromatography, and was obtained in yields of 1-2 mg/10(9) cells of material estimated to be about 70% pure. Pairs of rabbits were immunized with purified gp160 using either one of five different adjuvants or an immunostimulating complex. In all cases a substantial humoral immune response was obtained after boosting, including an activity that neutralized the homologous (BRU) isolate of HIV-1. In some cases, this activity also neutralized two distantly related isolates, SF2 and MN.
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Base de dados:
MEDLINE
Assunto principal:
Precursores de Proteínas
/
Anticorpos Anti-HIV
/
Produtos do Gene env
/
HIV-1
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article