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Study of injection bias in a simple hydrodynamic injection in microchip CE.
Gong, Maojun; Wehmeyer, Kenneth R; Stalcup, Apryll M; Limbach, Patrick A; Heineman, William R.
Afiliação
  • Gong M; Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221-0172, USA.
Electrophoresis ; 28(10): 1564-71, 2007 May.
Article em En | MEDLINE | ID: mdl-17447241
ABSTRACT
The electrokinetically pinched method is the most commonly used mode for sample injection in microchip capillary electrophoresis (microCE) due to its simplicity and well-defined sample volume. However, the limited injection volume and the electrophoretic bias of the pinched injection may limit its universal usage to specific applications. Several hydrodynamic injection methods in microCE have been reported; however, almost all claimed that their methods are bias-free without considering the dispensing bias. To investigate the dispensing bias, a simple hydrodynamic injection was developed in single-T and double-T glass microchips. The sample flow was produced by hydrostatic pressure generated by the liquid level difference between the sample reservoir and the other reservoirs. The reproducibility of peak area and peak area ratio was improved to a significant extent using large-surface reservoirs for the buffer reservoir and the sample waste reservoir to reduce the Laplace pressure effect. Without a voltage applied on the sample solution, the voltage-related sample bias was eliminated. The dispensing bias was analyzed theoretically and studied experimentally. It was demonstrated that the dispensing bias existed and could be reduced significantly by appropriately setting up the voltage configuration and by controlling the appropriate liquid level difference.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise de Injeção de Fluxo / Eletroforese em Microchip / Modelos Químicos Idioma: En Ano de publicação: 2007 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise de Injeção de Fluxo / Eletroforese em Microchip / Modelos Químicos Idioma: En Ano de publicação: 2007 Tipo de documento: Article