Footprinting: a method for determining the sequence selectivity, affinity and kinetics of DNA-binding ligands.
Methods
; 42(2): 128-40, 2007 Jun.
Article
em En
| MEDLINE
| ID: mdl-17472895
ABSTRACT
Footprinting is a simple method for assessing the sequence selectivity of DNA-binding ligands. The method is based on the ability of the ligand to protect DNA from cleavage at its binding site. This review describes the use of DNase I and hydroxyl radicals, the most commonly used footprinting probes, in footprinting experiments. The success of a footprinting experiment depends on using an appropriate DNA substrate and we describe how these can best be chosen or designed. Although footprinting was originally developed for assessing a ligand's sequence selectivity, it can also be employed to estimate the binding strength (quantitative footprinting) and to assess the association and dissociation rate constants for slow binding reactions.
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Pegada de DNA
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article