Short report: production of recombinant kinesin-related protein of Leishmania donovani and its application in the serodiagnosis of visceral leishmaniasis.
Am J Trop Med Hyg
; 76(5): 902-5, 2007 May.
Article
em En
| MEDLINE
| ID: mdl-17488913
ABSTRACT
To detect IgG antibody in the serodiagnosis of visceral leishmaniasis (VL), a recombinant antigen rK39, which is part of a Leishmania chagasi kinesin-related protein, has been used successfully and showed high sensitivity and specificity. We report production of a recombinant protein rKRP42, which is part of an L. donovani kinesin-related protein and a homolog of rK39, and its application in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of VL. When rKRP42 and rK39 were compared, amino acid sequence analysis showed 89.3% identity and 98.7% homology, with rKRP42 having 39 more amino acids than rK39. The ELISA using rKRP42 showed a sensitivity of 94.6% (70 positive samples among 74 from VL patients) and a specificity of 99.3% (148 negative samples among 149 samples from Japanese controls), whereas the sensitivity of the commercial rK39 dipstick test was 93.2% (69 positive samples among 74 from patients with VL). The rKRP42 is a promising new antigen in developing immunodiagnostic methods for VL.
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Base de dados:
MEDLINE
Assunto principal:
Leishmania donovani
/
Proteínas Recombinantes
/
Anticorpos Antiprotozoários
/
Proteínas de Protozoários
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Cinesinas
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Leishmaniose Visceral
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article