Quantitative single particle tracking of NGF-receptor complexes: transport is bidirectional but biased by longer retrograde run lengths.
FEBS Lett
; 581(16): 2905-13, 2007 Jun 26.
Article
em En
| MEDLINE
| ID: mdl-17543952
ABSTRACT
The retrograde transport of nerve growth factor (NGF) in neurite-like processes of living differentiated PC12 cells was studied using streptavidin-quantum dots (QDs) coupled to monobiotin-NGF. These reagents were active in differentiation, binding, internalization, and transport. Ten-35% of the QD-NGF-receptor complexes were mobile. Quantitative single particle tracking revealed a bidirectional step-like motion, requiring intact microtubules, with a net retrograde velocity of 0.054+/-0.020 microm/s. Individual runs had a mean velocity of approximately 0.15 microm/s at room temperature, and the run times were exponentially distributed. The photostability and brightness of QDs permit extended real-time analysis of individual QDbNGF- receptor complexes trafficking within neurites.
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Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
Receptor de Fator de Crescimento Neural
/
Complexos Multiproteicos
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article