Efficiency of a self-aminoacylating ribozyme: effect of the length and base-composition of its 3' extension.
RNA
; 13(8): 1191-7, 2007 Aug.
Article
em En
| MEDLINE
| ID: mdl-17556712
ABSTRACT
Variants of a previously described small self-aminoacylating ribozyme are tested in order to uncover the potentialities of a 3' extension responsible for the esterification. The base-composition and the length of this specific part of the ribozyme are investigated. Very short extensions can still reach the active site, reflecting the small persistence length of RNA. The yield of aminoacylation is particularly high for ribozymes with extensions made up of a poly-U, for which a maximum of efficiency is observed for a total length of about 10 nucleotides. A simple model describing the behavior of this region of the ribozyme can account for the data.
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Base de dados:
MEDLINE
Assunto principal:
RNA Catalítico
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article