Qualitative and comparative proteomic analysis of Xanthomonas campestris pv. campestris 17.
Proteomics
; 7(12): 2047-58, 2007 Jun.
Article
em En
| MEDLINE
| ID: mdl-17566974
ABSTRACT
The bacterium Xanthomonas campestris pathovar campestris (XCC) 17 is a local isolate that causes crucifer black rot disease in Taiwan. In this study, its proteome was separated using 2-DE and the well-resolved proteins were excised, trypsin digested, and analyzed by MS. Over 400 protein spots were analyzed and 281 proteins were identified by searching the MS or MS/MS spectra against the proteome database of the closely related XCC ATCC 33913. Functional categorization of the identified proteins matched 141 (50%) proteins to 81 metabolic pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. In addition, we performed a comparative proteome analysis of the pathogenic strain 17 and an avirulent strain 11A to reveal the virulence-related proteins. We detected 22 up-regulated proteins in strain 17 including the degrading enzymes EngXCA, HtrA, and PepA, which had been shown to have a role in pathogenesis in other bacteria, and an anti-host defense protein, Ohr. Thus, further functional studies of these up-regulated proteins with respect to their roles in XCC pathogenicity are suggested.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Xanthomonas campestris
/
Proteoma
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article