[Leukemic cell apoptosis induced by anti-human DR5 monoclonal antibody mDRA-6].

ABSTRACT

AIM: To investigate the apoptotic effect of anti-human DR5 (death receptor 5 of TRAIL) monoclonal antibody mDRA-6 on leukemic cells. METHODS: The morphological changes of leukemic cells were observed by fluorescence microscope. The cytotoxic and apoptotic effects of mDRA-6 on Jurkat, HL-60 and K562 cells were detected by MTT analysis and flow cytometry with Annexin V-FITC/PI staining. RESULTS: Chromatin condensation, budding and apoptotic bodies were observed in Jurkat and HL-60 cells treated by mDRA-6. Death and apoptosis of leukemic cells treated by mDRA-6 were increased, but the effect of mDRA-6 on K562 cells was not obvious. CONCLUSION: Apoptosis of leukemic cells can be induced by anti-human DR5 monoclonal antibody mDRA-6. Different leukemic cell lines are of different sensitivity to mDRA-6.