Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.
PLoS Biol
; 5(8): e192, 2007 Aug.
Article
em En
| MEDLINE
| ID: mdl-17622196
ABSTRACT
Transcription in mammalian nuclei is highly compartmentalized in RNA polymerase II-enriched nuclear foci known as transcription factories. Genes in cis and trans can share the same factory, suggesting that genes migrate to preassembled transcription sites. We used fluorescent in situ hybridization to investigate the dynamics of gene association with transcription factories during immediate early (IE) gene induction in mouse B lymphocytes. Here, we show that induction involves rapid gene relocation to transcription factories. Importantly, we find that the Myc proto-oncogene on Chromosome 15 is preferentially recruited to the same transcription factory as the highly transcribed Igh gene located on Chromosome 12. Myc and Igh are the most frequent translocation partners in plasmacytoma and Burkitt lymphoma. Our results show that transcriptional activation of IE genes involves rapid relocation to preassembled transcription factories. Furthermore, the data imply a direct link between the nonrandom interchromosomal organization of transcribed genes at transcription factories and the incidence of specific chromosomal translocations.
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Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
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Regulação da Expressão Gênica
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Proteínas Proto-Oncogênicas c-myc
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Cadeias Pesadas de Imunoglobulinas
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Genes Precoces
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Genes de Cadeia Pesada de Imunoglobulina
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article