Development of a genus-specific PCR assay for the molecular detection, confirmation and identification of Fusobacterium spp.
Br J Biomed Sci
; 64(2): 74-7, 2007.
Article
em En
| MEDLINE
| ID: mdl-17633142
ABSTRACT
A genus-specific polymerase chain reaction (PCR)-based assay is developed for the detection and identification of clinically relevant Fusobacterium species, including F. nucleatum and F. necrophorum. Two 16S ribosomal DNA (rDNA) primers, FUSO1 (forward primer 5'-GAG AGA GCT TTG CGT CC-3' [17-mer]) and FUSO 2 (reverse primer 5'-TGG GCG CTG AGG TTC GAC -3' [18-mer]) are designed to target conserved regions of the 16S rDNA gene for Fusobacterium spp. Subsequent proof-of-principle studies employing this assay detected Fusobacterium spp. in the faeces of eight (10%) out of 80 patients with suspected gastrointestinal infection. This assay may be used for the genus-specific detection of Fusobacterium spp. from clinical specimens and for subsequent species identification.
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Base de dados:
MEDLINE
Assunto principal:
DNA Ribossômico
/
Fusobacterium
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article