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Comparison of different cell lines and incubation times in the isolation by the shell vial culture of human metapneumovirus from pediatric respiratory samples.
Reina, Jordi; Ferres, Francesc; Alcoceba, Eva; Mena, Aina; de Gopegui, Enrique Ruiz; Figuerola, Joan.
Afiliação
  • Reina J; Virology Unit, Clinical Microbiology Service, University Hospital Son Dureta, Andrea Doria 55, 07014 Palma de Mallorca, Spain. jreina@hsd.es
J Clin Virol ; 40(1): 46-9, 2007 Sep.
Article em En | MEDLINE | ID: mdl-17644404
We report a prospective study concerning the efficacy of LLC-MK2 (continuous monkey kidney cell), Hep-2, MDCK (Madin-Darby Canine Kidney), Vero and MRC-5 cell lines, by shell vial assay, and incubation time in the isolation of hMPV from pediatric respiratory samples. The overall sensitivity of the cell lines studied were: 100% for the LLC-MK2, 68.7% for the Hep-2, 28.1% for the Vero, 3.1% for the MDCK and 0% for the MRC-5. Only one strain (3.1%) showed growth in the four cell lines studied and 10 (31.2%) strains only grew in the LLC-MK2 cell line. The analysis of incubation times showed that only 14 strains (43.7%) were able to grow after 3 days of incubation, while all strains (100%) showed growth after 5 days. The use of shell vials with commercial LLC-MK2 cells could be a method for isolating hMPV from respiratory samples in the pediatric population.
Assuntos
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Base de dados: MEDLINE Assunto principal: Infecções Respiratórias / Cultura de Vírus / Nasofaringe / Infecções por Paramyxoviridae / Metapneumovirus Idioma: En Ano de publicação: 2007 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Infecções Respiratórias / Cultura de Vírus / Nasofaringe / Infecções por Paramyxoviridae / Metapneumovirus Idioma: En Ano de publicação: 2007 Tipo de documento: Article