Nuclear import of c-Jun is mediated by multiple transport receptors.
J Biol Chem
; 282(38): 27685-92, 2007 Sep 21.
Article
em En
| MEDLINE
| ID: mdl-17652081
c-Jun and c-Fos are major components of the transcriptional complex AP-1. Here, we investigate the nuclear import pathway(s) of the transcription factor c-Jun. c-Jun bound specifically to the nuclear import receptors importin beta, transportin, importin 5, importin 7, importin 9, and importin 13. In digitonin-permeabilized cells, importin beta, transportin, importin 7, and importin 9 promoted efficient import of c-Jun into the nucleus. Importin alpha, by contrast, inhibited nuclear import of c-Jun in vitro. A single basic region preceding the leucine zipper of c-Jun functions as a nuclear localization signal (NLS) and was required for interaction with all tested import receptors. In vivo, nuclear import of a c-Jun reporter protein lacking the leucine zipper strictly depended on this NLS. In a leucine zipper-dependent manner, c-Jun with mutations in its NLS was still imported into the nucleus in a complex with endogenous leucine zipper proteins or, for example, with cotransfected c-Fos. Together, these results explain the highly efficient nuclear import of the transcription factor c-Jun.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Proto-Oncogênicas c-jun
/
Proteínas Proto-Oncogênicas c-fos
/
Transporte Ativo do Núcleo Celular
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Carioferinas
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article