Effects of hyperbaric oxygen and platelet derived growth factor on medial collateral ligament fibroblasts.
Undersea Hyperb Med
; 34(3): 181-90, 2007.
Article
em En
| MEDLINE
| ID: mdl-17672174
ABSTRACT
PURPOSE:
This study investigated hyperbaric oxygen (HBO2) and platelet-derived growth factor-BB (PDGF-BB) to determine their combined effects on fibroblasts from rabbit medial collateral ligament (MCL).METHOD:
Cells were divided into four groups (I) Control, (II) HBO2 treatment, (III) PDGF-BB treatment and (IV) HBO2 combined with PDGF-BB treatment. All hyperoxic cells were exposed to 100% O2 at 2.5 atmospheres absolute (ATA) in a hyperbaric chamber for 120 minutes per 48 hours. Measurement of cell growth was based on increase in cell number. Cell cycle modulations were analyzed by fluorescence-activated cell sorter (FACS). Quantity of Type I and Type III collagen was determined by western blotting and image analyzer.RESULTS:
Treatment doses of HBO2 alone or PDGF-bb alone dependently increased cell growth. A combination of HBO2 treatment plus PDGF-bb treatment had an additive effect on cell growth in comparison with HBO2 treatment alone or PDGF-bb treatment alone. FACS analysis revealed that HBO2 alone, PDGF-bb alone and PDGF-bb plus HBO2 treatment increase the percentage of cells accumulated in S-phase. Western blotting analysis revealed that Type III collagen content was decreased significantly after HBO2 treatment alone or HBO2 plus PDGF-bb treatment but not in PDGF-bb treatment alone. In contrast, although Type I collagen content was increased after HBO2 treatment, the increase in Type I collagen (increase /original) was not statistically significant.CONCLUSION:
HBO2 or HBO2 plus PDGF-bb treatment decreases the Type III collagen/Type I collagen content, which could result in mechanically stronger collagen fibrils. We propose HBO2 therapy as a potentially effective treatment for MCL healing.
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Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Fator de Crescimento Derivado de Plaquetas
/
Ligamento Colateral Médio do Joelho
/
Fibroblastos
/
Oxigenoterapia Hiperbárica
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article