Production and characterization of monoclonal antibody against recombinant human erythropoietin.
Biomed Environ Sci
; 20(3): 184-8, 2007 Jun.
Article
em En
| MEDLINE
| ID: mdl-17672207
ABSTRACT
OBJECTIVE:
To produce specific monoclonal antibody (mAb) against recombinant human erythropoietin (rHuEPO) for development of highly efficient methods for erythropoietin detection in biological fluids.METHODS:
rHuEPO was covalently coupled with bovine serum albumin (BSA) and the conjugate was used to immunize mice to produce specific mAb against rHuEPO based on hybridoma technology. The obtained F3-mAb was characterized by enzyme-linked immunosorbent assay (ELISA), SDS-PAGE and Western blot.RESULTS:
The isotype of F3-mAb was found to be IgM with an affinity constant of 2.1 x 10(8) L/mol. The competitive ELISA using the obtained IgM showed a broader linear range and lower detection limit compared with previous work.CONCLUSIONS:
The modification of rHuEPO was proved to be successful in generating required specific mAb with high avidity to rHuEPO.
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Base de dados:
MEDLINE
Assunto principal:
Eritropoetina
/
Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article