Germline NBS1 mutations in families with aggregation of Breast and/or ovarian cancer from north-east Poland.
Anticancer Res
; 27(4C): 3015-8, 2007.
Article
em En
| MEDLINE
| ID: mdl-17695489
ABSTRACT
BACKGROUND:
NBS1 gene, which product participates in DNA repair, has been postulated to be a susceptibility factor for a number of types of cancer, including breast cancer. The carrier frequency of the 657del5 and I171V NBS1 gene mutations among Polish patients with familial breast and/or ovarian cancer was compared with that of randomly selected newborns. PATIENTS ANDMETHODS:
Using allele-specific amplification-polymerase chain reaction (ASAPCR) and restriction fragment length polymorphismpolymerase chain reaction (RFLP-PCR) techniques, blood samples were analysed from 250 patients with breast or/and ovarian cancer and a total number of 4,000 for 657del5 mutation and 1,300 for I1171V mutation controls.RESULTS:
Although an increased frequency of both mutations in cancer cases - 0.8% of 657del5 and 2.4% of I171V, compared to controls - 0.52% and 1.38%, respectively, was found, the differences were not statistically significant.CONCLUSION:
Our results indicate that NBS1 mutations do not contribute significantly to breast or ovarian cancer development.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ovarianas
/
Neoplasias da Mama
/
Proteínas Nucleares
/
Mutação em Linhagem Germinativa
/
Proteínas de Ciclo Celular
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article