Intracellular calcium chelators and oxidant-induced renal proximal tubule cell death.
J Biochem Toxicol
; 6(4): 299-303, 1991.
Article
em En
| MEDLINE
| ID: mdl-1774771
ABSTRACT
The effect of intracellular calcium chelators on rabbit renal proximal tubule (RPT) cell death induced by t-butyl hydroperoxide (TBHP) and H2O2 was examined. Preincubation of RPT suspensions with 50 microM QUIN 2/AM completely prevented TBHP (0.5 mM) and H2O2 (2 mM) induced cell death [i.e., release of lactate dehydrogenase (LDH)]. QUIN 2/AM, BAPTA/AM, EGTA/AM, and FURA 2/AM, at 5 microM, decreased LDH release (at 6 hr) from 41% to 4%, 21%, 26%, and 33%, and decreased lipid peroxidation (at 1 hr) from 1.0 to 0.1, 0.4, 0.6, and 0.8 nmol MDA/mg protein, respectively, after TBHP exposure. Since oxidant-induced lipid peroxidation and cell death are iron-dependent in this model, these results suggest that the intracellular calcium chelators inhibit cell death by chelating iron.
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Base de dados:
MEDLINE
Assunto principal:
Quelantes
/
Cálcio
/
Túbulos Renais Proximais
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article