[Screening of polypeptides interacting with Rel homology domain of NF-kappaB p50 subunit].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 23(9): 804-6, 2007 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-17825223
ABSTRACT
AIM:
To obtain the polypeptides interacting with NF-kappaB p50 Rel homology domain (RHD) by yeast two-hybrid technique.METHODS:
Using NF-kappaB p50 RHD as bait, the polypeptides interacting with NF-kappaB p50 RHD were screened from a 16-peptide cDNA library by yeast two-hybrid technique. The false positive clones were screened out but the positive clone were identified by beta-GAL assay, yeast mating, and one to one yeast two-hybrid method.RESULTS:
8 positive clones were obtained. The gene sequences of the eight polypeptides were different from each other and their homologous proteins were not found in GenBank.CONCLUSION:
8 novel polypeptides interacting with NF-kappaB p50 RHD were obtained, but their functions need to be validated in further experiments.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Homologia de Sequência de Aminoácidos
/
Subunidade p50 de NF-kappa B
/
Fator de Transcrição RelA
/
Fator de Transcrição RelB
Idioma:
Zh
Ano de publicação:
2007
Tipo de documento:
Article