Cell surface charge and sugar residues of Crithidia fasciculata and Crithidia luciliae.
Microbios
; 68(275): 87-96, 1991.
Article
em En
| MEDLINE
| ID: mdl-1784253
ABSTRACT
The surface charge of Crithidia fasciculata and Crithidia luciliae was analysed by measurement of the zeta-potential and labelling of the protozoan surface with cationized ferritin particles. Both trypanosomatids have a net negative surface charge, with a zeta-potential of -10.39 mV and -11.12 mV for C. luciliae and C. fasciculata, respectively. Enzyme treatment showed that phosphate groups, but not sialic acid, significantly contributed to the negative surface charge. Lectin-induced agglutination was used to analyse the presence of surface-exposed carbohydrates in C. fasciculata and C. luciliae. The cells did not agglutinate when incubated in the presence of lectins which recognized L-fucose, N-acetyl-D-glucosamine and sialic acid. However, lectins which bind to N-acetyl-D-galactosamine, D-galactose and D-mannose agglutinated both protozoa.
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Base de dados:
MEDLINE
Assunto principal:
Carboidratos
/
Crithidia fasciculata
/
Crithidia
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article