Thiol redox state and oxidative stress affect sclerotial differentiation of the phytopathogenic fungi Sclerotium rolfsii and Sclerotinia sclerotiorum.
J Appl Microbiol
; 104(1): 42-50, 2008 Jan.
Article
em En
| MEDLINE
| ID: mdl-17850300
ABSTRACT
AIMS:
To investigate the involvement of oxidative stress and thiol redox state (TRS) in sclerotial differentiation of Sclerotium rolfsii and Sclerotinia sclerotiorum. METHODS ANDRESULTS:
Oxidative stress in these fungi was assessed by lipid peroxidation, which was higher in comparison with their nonsclerotiogenic counterpart strains. TRS [measured as glutathione (GSH) and cysteine] was associated with oxidative stress and differentiation using the TRS modulator and antioxidant Nu-acetylcysteine (AcCSH) and the GSH biosynthesis inducer and inhibitor l-2-oxo-thiazolidine-4-carboxylate and L-buthionine-S,R-sulphoximine (BSO) respectively. Differentiation and oxidative stress was decreased by AcCSH in both fungi. The decrease of differentiation by BSO was not associated with oxidative stress in these fungi.CONCLUSIONS:
Differentiation and oxidative stress in both fungi depends on the availability of antioxidant noncytotoxic -SH groups and is not depended on any direct antioxidant role of GSH and its precursor cysteine. SIGNIFICANCE AND IMPACT OF THE STUDY This study helps to understand the mechanism(s) of sclerotial differentiation in these agriculturally important phytopathogenic fungi and proposes that AcCSH can be used as potent fungicide by (i) acting as growth inhibiting cytotoxic oxidant and (ii) sustaining these fungi in their undifferentiated hyphal stage where they are vulnerable to degradation by soil micro-organisms.
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
/
Ascomicetos
/
Compostos de Sulfidrila
/
Basidiomycota
/
Proteínas Fúngicas
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article