A comparison of mitochondrial respiratory function of Tibet chicken and Silky chicken embryonic brain.

ABSTRACT

The Tibet chicken lives in high altitude and has adapted itself well to hypoxia. The Silky chicken is a lowland chicken from Jiangxi province of China. The objective of the present study was to investigate whether there were any differences in brain mitochondrial respiratory function between Tibet chicken and Silky chicken embryos incubated in a normoxic (21% oxygen concentration) or simulated hypoxic (13% O(2)) hatchibator. Brain mitochondria of chicken embryos were prepared by differential centrifugation on d 16 of incubation. The respiratory control ratio (RCR) and the adenosine 5'-diphosphate oxygen ratio (ADP/O) were determined polarographically. The complex I activity was measured with an ultraviolet spectrophotometer by following the oxidation of the reduced state of beta-nicotinamide adenine dinucleotide. Under the normoxic incubation condition, there were no significant differences in the RCR, the ADP/O, and the activity of complex I between embryonic brain mitochondria of the 2 breeds. Under the hypoxic incubation condition, the ADP/O in brain mitochondria of embryos from the 2 breeds were identical. Also under hypoxic conditions the RCR in brain mitochondria of Tibet chicken embryos was higher (P < 0.05) than in Silky chicken embryos when brain mitochondria were provided with glutamate-malate, but no significant difference was found in the RCR with succinate as an energy substrate. The complex I activity of Silky chicken embryos was higher than that of Tibet chicken embryos when they were incubated in the hypoxic hatchibator (P < 0.01). In conclusion, the results show that under simulated hypoxic incubation conditions electron transport in brain mitochondria of Tibet chicken embryos was more tightly coupled than that of lowland chicken (Silky chicken) embryos with glutamate-malate as energy substrate, which was associated with the difference in the activity of complex I between embryonic brains of the 2 breeds. This work will provide reference for future studies on the association of mitochondrial respiratory function with the adaptation to hypoxia.