Identification of candidate secreted factors involved in trigeminal placode induction.
Dev Dyn
; 236(10): 2925-35, 2007 Oct.
Article
em En
| MEDLINE
| ID: mdl-17879314
ABSTRACT
Cranial ectodermal placodes are critical for normal development of the peripheral nervous system of the head. However, many aspects of the molecular and tissue interactions involved in their induction have yet to be elucidated. The trigeminal placode is induced by an unidentified secreted factor(s) from the dorsal neural tube. To determine candidates that may be involved in this induction process, we have performed reverse transcriptase-polymerase chain reaction (RT-PCR) and whole-mount in situ hybridization to screen for receptors expressed by uninduced presumptive trigeminal level ectoderm. We have found that receptors for fibroblast growth factors, insulin-like growth factors, platelet-derived growth factors, Sonic hedgehog, the transforming growth factor-beta superfamily, and Wnts all are expressed in patterns consistent with a role in trigeminal placode formation. This RT-PCR screen for candidate receptors expressed in presumptive trigeminal ectoderm is the first systematic screen to identify potential interactions underlying induction of the trigeminal placode and represents a critical step for understanding this complex process.
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Base de dados:
MEDLINE
Assunto principal:
Gânglio Trigeminal
/
Receptores de Fatores de Crescimento
/
Receptores de Superfície Celular
/
Ectoderma
/
Indução Embrionária
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article