Digital NFATc2 activation per cell transforms graded T cell receptor activation into an all-or-none IL-2 expression.
PLoS One
; 2(9): e935, 2007 Sep 26.
Article
em En
| MEDLINE
| ID: mdl-17895976
ABSTRACT
The expression of interleukin-2 (IL-2) is a key event in T helper (Th) lymphocyte activation, controlling both, the expansion and differentiation of effector Th cells as well as the activation of regulatory T cells. We demonstrate that the strength of TCR stimulation is translated into the frequency of memory Th cells expressing IL-2 but not into the amount of IL-2 per cell. This molecular switch decision for IL-2 expression per cell is located downstream of the cytosolic Ca2+ level. Here we show that in a single activated Th cell, NFATc2 activation is digital but NF-kappaB activation is graded after graded T cell receptor (TCR) signaling. Subsequently, NFATc2 translocates into the nucleus in an all-or-none fashion per cell, transforming the strength of TCR-stimulation into the number of nuclei positive for NFATc2 and IL-2 transcription. Thus, the described NFATc2 switch regulates the number of Th cells actively participating in an immune response.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Receptores de Antígenos de Linfócitos T
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Interleucina-2
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Linfócitos T Auxiliares-Indutores
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Fatores de Transcrição NFATC
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article