Rapid screening of chromatography resins for the purification of proteins.
Methods Mol Biol
; 389: 99-106, 2007.
Article
em En
| MEDLINE
| ID: mdl-17951637
ABSTRACT
With an ever increasing number of proteins being expressed in the Pichia system, there is a growing need to rapidly develop scalable and robust purification schemes. This chapter describes a high-throughput method to screen for the optimal chromatography conditions and resin to capture and release a protein secreted by Pichia pastoris. The method involves a chromatography matrix involving four resins (Q-Sepharose, DEAE-Sepharose, SP-Sepharose, and CMSepharose), 4 pHs from 5.0 to 8.0, and 3 NaCl concentrations. The method was tested on three proteins and found to be reproducible and easily scalable.
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Base de dados:
MEDLINE
Assunto principal:
Pichia
/
Resinas Sintéticas
/
Proteínas Fúngicas
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article