Differentially expressed genes under cold acclimation in Physcomitrella patens.
J Biochem Mol Biol
; 40(6): 986-1001, 2007 Nov 30.
Article
em En
| MEDLINE
| ID: mdl-18047796
ABSTRACT
Cold acclimation improves freezing tolerance in plants. In higher plants, many advances have been made toward identifying the signaling and regulatory pathways that direct the low-temperature stress response; however, similar insights have not yet been gained for simple nonvascular plants, such as bryophytes. To elucidate the pathways that regulate cold acclimation in bryophytes, we used two PCR-based differential screening techniques, cDNA amplified fragment length polymorphism (cDNA-AFLP) and suppression subtractive hybridization (SSH), to isolate 510 ESTs that are differentially expressed during cold acclimation in Physcomitrella patens. We used realtime RT-PCR to further analyze expression of 29 of these transcripts during cold acclimation. Our results show that cold acclimation in the bryophyte Physcomitrella patens is not only largely similar to higher plants but also displays distinct differences, suggests significant alteration during the evolution of land plants.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Genes de Plantas
/
Bryopsida
/
Aclimatação
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article