Cell-cycle regulation of cohesin stability along fission yeast chromosomes.
EMBO J
; 27(1): 111-21, 2008 Jan 09.
Article
em En
| MEDLINE
| ID: mdl-18079700
ABSTRACT
Sister chromatid cohesion is mediated by cohesin, but the process of cohesion establishment during S-phase is still enigmatic. In mammalian cells, cohesin binding to chromatin is dynamic in G1, but becomes stabilized during S-phase. Whether the regulation of cohesin stability is integral to the process of cohesion establishment is unknown. Here, we provide evidence that fission yeast cohesin also displays dynamic behavior. Cohesin association with G1 chromosomes requires continued activity of the cohesin loader Mis4/Ssl3, suggesting that repeated loading cycles maintain cohesin binding. Cohesin instability in G1 depends on wpl1, the fission yeast ortholog of mammalian Wapl, suggestive of a conserved mechanism that controls cohesin stability on chromosomes. wpl1 is nonessential, indicating that a change in wpl1-dependent cohesin dynamics is dispensable for cohesion establishment. Instead, we find that cohesin stability increases at the time of S-phase in a reaction that can be uncoupled from DNA replication. Hence, cohesin stabilization might be a pre-requisite for cohesion establishment rather than its consequence.
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Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
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Proteínas Nucleares
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Proteínas Cromossômicas não Histona
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Cromossomos Fúngicos
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Ciclo Celular
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Proteínas de Ciclo Celular
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article