Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity.
Opt Lett
; 24(24): 1832-4, 1999 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-18079946
ABSTRACT
We describe a laser-scanning two-photon fluorescence microscope that is capable of observing single molecules with excellent temporal resolution and three-dimensional spatial resolution. To demonstrate the capabilities of the instrument we present single-molecule fluorescence data obtained in several different scanning modes. In addition, a polarization-sensitive detection scheme can provide detailed three-dimensional information about the orientations of molecules in any of these scanning modes.
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Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article