Fibronectin suppresses lipopolysaccharide-induced liver damage and promotes the cytoprotection abilities of hepatocyte-like cells derived from human bone marrow mesenchymal stem cells.
Transplant Proc
; 39(10): 3444-5, 2007 Dec.
Article
em En
| MEDLINE
| ID: mdl-18089402
ABSTRACT
We isolated mesenchymal stem cells (MSCs) from adult human bone marrow. By using reverse-transcription polymerase chain reactions, we confirmed that MSCs possessed the potential to differentiate into hepatocyte-like cells (MSC-HLCs) with the expression of hepatocyte-specific marker genes. We further observed that fibronectin (FN) treatment significantly inhibited lipopolysaccharide (LPS)-induced apoptotic activities in FN-treated MSC-HLCs, as detected by caspase 3 enzyme-linked immunosorbent (ELISA) and terminal dUTP nick-end labeling (TUNEL) assays (P<.05). The FN-treated MSC-HLCs were transplanted into SCID mice with or without LPS injection. This study demonstrated that FN treatment improved liver function repair and survival rates among LPS-treated SCID mice.
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Base de dados:
MEDLINE
Assunto principal:
Lipopolissacarídeos
/
Fibronectinas
/
Hepatócitos
/
Transplante de Células-Tronco Mesenquimais
/
Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article