Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein.
Proc Natl Acad Sci U S A
; 105(1): 157-61, 2008 Jan 08.
Article
em En
| MEDLINE
| ID: mdl-18165314
ABSTRACT
The V2 protein of tomato yellow leaf curl geminivirus (TYLCV) functions as an RNA-silencing suppressor that counteracts the innate immune response of the host plant. The host-cell target of V2, however, remains unknown. Here we show that V2 interacts directly with SlSGS3, the tomato homolog of the Arabidopsis SGS3 protein (AtSGS3), which is known to be involved in the RNA-silencing pathway. SlSGS3 genetically complemented an AtSGS3 mutation and restored RNA silencing, indicating that SlSGS3 is indeed a functional homolog of AtSGS3. A point mutant of V2 that is unable to bind SlSGS3 also lost its ability to suppress RNA silencing, suggesting a correlation between the V2-SlSGS3 interaction in planta and the suppressor activity of V2.
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1
Base de dados:
MEDLINE
Assunto principal:
Interferência de RNA
/
Begomovirus
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article