Simultaneous clinical monitoring of lactic acid, pyruvic acid and ketone bodies in plasma as methoxime/tert-butyldimethylsilyl derivatives by gas chromatography-mass spectrometry in selected ion monitoring mode.
Biomed Chromatogr
; 22(5): 450-3, 2008 May.
Article
em En
| MEDLINE
| ID: mdl-18254151
ABSTRACT
Simultaneous determination of lactic acid, pyruvic acid, 3-hydroxybutyric acid and acetoacetic acid for clinical monitoring of lactic acidosis and ketone body formation in human plasma (20 microL) was performed by gas chromatography-mass spectrometry in selected ion monitoring (SIM) mode after generating methoxime/tert-butyldimethylsilyl derivatives. All of the targeted carboxylic acids were detected by characteristic fragment ions, which permitted sensitive and selective identification in the presence of co-extracted free fatty acids and other acidic metabolites at much higher levels. The method was linear (r>or=0.9991), reproducible (% relative standard deviation=1.2-5.8), and accurate (% relative error=-7.2-7.6), with detection limits of 0.05-1.7 ng/mL. This rapid, accurate and selective method using minimal plasma samples (20 microL) is useful in the clinical monitoring of lactic acidosis and ketone body formation in plasma.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oximas
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Compostos de Organossilício
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Ácido Láctico
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Ácido Pirúvico
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Corpos Cetônicos
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Cromatografia Gasosa-Espectrometria de Massas
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article