Protein synthesis elongation factor Tu present in spores of Streptomyces coelicolor can be phosphorylated in vitro by the spore protein kinase.
Folia Microbiol (Praha)
; 52(5): 471-8, 2007.
Article
em En
| MEDLINE
| ID: mdl-18298043
ABSTRACT
In vitro phosphorylation of EF-Tu was shown in cell-free extract from dormant spores of Streptomyces coelicolor by a protein kinase present in spores. EF-Tu phosphorylation was observed on both intrinsic S. coelicolor factor and externally added purified EF-Tu from S. aureofaciens, on two isoforms. Putative serine and threonine residues as potential phosphorylation targets were determined in primary sequence and demonstrated on 3D structure model of EF-Tu.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Quinases
/
Esporos Bacterianos
/
Fator Tu de Elongação de Peptídeos
/
Streptomyces coelicolor
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article