[Determination of iridoids and triterpenes in herb of Swertia pseudochinesis by RP-HPLC].
Zhongguo Zhong Yao Za Zhi
; 32(23): 2494-6, 2007 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-18330241
ABSTRACT
OBJECTIVE:
To establish a RP-HPLC method for the determination of swertiamarin, sweroside, gentiopicrin and oleanolic acid in different parts of Swertia pseudochinesis.METHOD:
A Zorbax SB-C18 (4.6 mm x 250 mm, 5 microm) column was used with acetonitrile-water (1090) and methnol-water(8614) at detection wavelengths of 238 nm, 246 nm, 274 nm and 207 nm for swertiamarin, sweroside, gentiopicrin and oleanolic acid respectively. The flow rate was 1.0 mL x min(-1) and the column temperature was 25 degrees C.RESULT:
All of the compounds were based--isolated. The linear ranges of swertiamarin, sweroside, gentiopicrin and oleanolic acid were 0.068 9-0.344 4(r = 0.999 2) , 0.001 1-0.014 0 (r2 = 0. 999 8), 0.001 1-0.013 4 (r2 = 0.999 9) and 0.001 1-0.008 8 mg x mL(-1) (r2 = 0. 999 6), respectively.CONCLUSION:
The method is simple and accurate, which can be used for quality control of S. pseudochinesis.
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Base de dados:
MEDLINE
Assunto principal:
Triterpenos
/
Cromatografia Líquida de Alta Pressão
/
Swertia
/
Iridoides
Idioma:
Zh
Ano de publicação:
2007
Tipo de documento:
Article