Determination of individual vitamin B(6) compounds based on enzymatic conversion to 4-pyridoxolactone.
J Nutr Sci Vitaminol (Tokyo)
; 54(1): 18-24, 2008 Feb.
Article
em En
| MEDLINE
| ID: mdl-18388403
ABSTRACT
A determination method for individual natural vitamin B(6) compounds was developed. The vitamin B(6) compounds were specifically converted into 4-pyridoxolactone (PAL), a highly fluorescent compound, through a combination of enzymatic reactions and HCl-hydrolysis. PAL was then determined by HPLC. Pyridoxal was completely oxidized to PAL with pyridoxal 4-dehydrogenase (PLDH). Pyridoxine and pyridoxamine were totally converted into PAL through a coupling reaction involving pyridoxine 4-oxidase and PLDH, and one involving pyridoxamine-pyruvate aminotransferase and PLDH, respectively. The 5'-phosphate forms and pyridoxine-beta-glucoside were hydrolyzed with HCl, and then determined as their free forms. Pyridoxine 5'-phosphate and pyridoxine-beta-glucoside were not separately determined here. Three food samples were analyzed by this method.
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Base de dados:
MEDLINE
Assunto principal:
Ácido Piridóxico
/
Complexo Vitamínico B
/
Oxirredutases do Álcool
/
Análise de Alimentos
/
Transaminases
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article