Thyroid transcription factor-1 exhibits osmosensitive transcription in brain-derived cell lines.
Biochem Biophys Res Commun
; 370(3): 468-72, 2008 Jun 06.
Article
em En
| MEDLINE
| ID: mdl-18395010
ABSTRACT
Thyroid transcription factor-1 (TTF-1) belongs to the Nkx family of homeodomain-containing proteins and regulates expression of several important genes in the brain. Our previous studies showed that TTF-1 plays an important role in water homeostasis in the subfornical organ of rats and is involved in cerebrospinal fluid formation by regulation of aquaporin-1 transcription in the choroid plexus. In this study, we examined changes in TTF-1 transcription in response to hypertonicity using promoter assays. TTF-1 was synthesized in several osmosensitive regions of the rat brain. TTF-1 promoter activity was diminished by treatment with hypertonic solutions in a time- and dose-dependent manner in brain-derived cell lines. Additionally, TTF-1 was involved in the regulation of angiotensinogen (Aogen) transcription under a hyperosmotic condition through specific binding domains in the Aogen promoter. These results suggest a possible role of TTF-1 in brain fluid homeostasis in response to changes in the osmotic environment.
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Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Encéfalo
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Proteínas Nucleares
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Angiotensinogênio
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Regulação da Expressão Gênica
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article