In vitro and in vivo analysis of neurotrophin-3 activation of Arf6 and Rac-1.
Methods Enzymol
; 438: 171-83, 2008.
Article
em En
| MEDLINE
| ID: mdl-18413248
ABSTRACT
Arf GTP-binding proteins and Rho-family GTPases play key roles in regulating membrane remodeling and cytoskeletal reorganization involved in cell movement. Several studies have implicated neurotrophins and their receptors as upstream activators of these small GTP-binding proteins, however, the mechanisms and the cell type specificity of this neurotrophin activity are still under investigation. Here we describe the rationale and protocols used for the dissection of an NT3 activated pathway that leads to the specific activation of Arf6 and Rac1.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fatores de Ribosilação do ADP
/
Proteínas rac1 de Ligação ao GTP
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Neurotrofina 3
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Neurônios
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article