Immortalizing central nervous system cells.
Curr Protoc Neurosci
; Chapter 3: Unit 3.7, 2001 May.
Article
em En
| MEDLINE
| ID: mdl-18428471
ABSTRACT
This unit presents methods for isolating clonal, neural-derived cell lines. One approach for isolating such neural cell lines involves a replication-deficient retrovirus encoding a specific oncogene and a selectable marker which are used to infect dissociated CNS cells dissected at a developmental stage at which the cell population of interest has not undergone its terminal mitotic division. Also presented is a method for cloning by limiting dilution, which may be necessary to obtain a pure population of cells. Following growth under appropriate selection conditions, clones are isolated and tested for their ability to differentiate with the desired phenotypic properties. A method is also provided for coating tissue culture dishes, which is necessary for successful culture of CNS neurons.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Linhagem Celular Transformada
/
Sistema Nervoso Central
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Técnicas de Cultura de Células
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Neurônios
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article