Proteomic techniques identify urine proteins that differentiate patients with interstitial cystitis from asymptomatic control subjects.
Am J Obstet Gynecol
; 198(5): 553.e1-6, 2008 May.
Article
em En
| MEDLINE
| ID: mdl-18455532
ABSTRACT
OBJECTIVE:
The purpose of this study was to identify differences in urine proteins between patients with interstitial cystitis (IC) and asymptomatic control (AC) subjects with the use of proteomic techniques. STUDYDESIGN:
Nine patients with IC and their age-, race-, and sex-matched AC subjects volunteered a urine specimen. Urine proteins were separated with the use of 2-dimensional polyacrylamide gels. Differing proteins underwent digestion and matrix-assisted laser desorption ionization-time of flight mass spectrometry. Computer-assisted data analysis was used to identify the corresponding protein. Differences in urine protein responses between patients with IC and AC subjects were evaluated by the Mann-Whitney U test to account for the nonnormal frequency distribution of the parameter estimate or chi-square when data were bimodal.RESULTS:
Four proteins differed significantly between patients with IC and AC subjects. The AC subjects had a greater concentration of a uromodulin (P = .019) and two kininogens (P = .023, .046). The patients with IC had a greater concentration of inter-alpha-trypsin inhibitor heavy chain H4 (P = .019).CONCLUSION:
These urine protein isoforms may be biomarkers for IC.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Urina
/
Proteínas
/
Cistite Intersticial
/
Proteômica
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article