Clonal CD8+ TCR-Vbeta expanded populations with effector memory phenotype in Churg Strauss syndrome.
Clin Immunol
; 128(1): 94-102, 2008 Jul.
Article
em En
| MEDLINE
| ID: mdl-18502180
ABSTRACT
Churg Strauss Syndrome (CSS) is a systemic vasculitis in which oligoclonal T cell expansions might be involved in the pathogenesis. Combined analysis of TCR-Vbeta expression profile by flow cytometry and of TCR gene rearrangement by heteroduplex PCR was used to detect and characterize T cell expansions in 8 CSS patients, 10 asthmatics and 42 healthy subjects. In all CSS patients one or two Vbeta families were expanded among CD8+ cells, with an effector memory phenotype apt to populate tissues and inflammatory sites. Heteroduplex PCR showed the presence of one or more clonal TCR rearrangements, which reveals monoclonal or oligoclonal T cells subpopulations. After purification with a Vbeta specific monoclonal antibody, each CD8+/Vbeta+ expanded family showed a single TCR rearrangement, clearly suggestive of monoclonality. All CD8+ expansions were detectable throughout the disease course. TCR-Vbeta expanded or deleted populations were not observed in asthmatic patients. Clonal CD8+/Vbeta+ T cell expansions might be useful as a disease marker.
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Base de dados:
MEDLINE
Assunto principal:
Síndrome de Churg-Strauss
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Subpopulações de Linfócitos T
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Receptores de Antígenos de Linfócitos T alfa-beta
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Linfócitos T CD8-Positivos
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Memória Imunológica
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article